Journal: Materials Today Bio
Article Title: Multifunctional hydrogel promotes rotator cuff healing through anti-inflammation and vascularization
doi: 10.1016/j.mtbio.2025.102016
Figure Lengend Snippet: Evaluation of the vascularization potential of the LA-CMCS-OHA hydrogel in vitro . (A, B) VEGFA protein levels were analyzed via western blot and semi-quantification. (C) VEGF levels were quantitatively measured using ELISA. (D, E) HUVEC proliferation rates were assessed through EDU analysis of CMCS-OHA and LA-CMCS-OHA hydrogels. scale bar: 10 μm. (F, G) CD31 protein levels were analyzed using immunofluorescence and semi-quantification. scale bar: 10 μm. (H) CD31 mRNA levels were determined via RT-qPCR. (I–L) Tube formation assays were conducted at 3 and 5 h to evaluate differences in tube formation, total length, and the number of junctions among groups. scale bar: 10 μm.
Article Snippet: Coronal sections were prepared for immunological examination, including immunofluorescence staining for iNOS, CD206, CD31, and α-SMA (Servicebio, GB111364-100, 1:500); immunohistochemistry for IL-6, TNF-α, and IL-10; and histopathological examination using H&E, Toluidine blue, and Masson's trichrome staining.
Techniques: In Vitro, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Quantitative RT-PCR